Biochemical equipment

Microbiology:

• Biostat A fermentation station (2x5L) (Sartorious stedim)

• 2x5L fermentor (Control flow)

• Incubator with a temperature regulator and shaker (Binder)

• MaxQ ™ 6000 Thermo Scientific ™ shaker incubator (2x)

• Shaking incubator New Brunswick Excella E24R

• Two incubated shakers LabCampanion (ISS-4075R)

• Autoclave Tuttnauer D-line 5075 ELV

• Sonics Vibra-Cell VCX500 ultrasonic disintegrator

• High-pressure laboratory homogenizer EmulsiFlex-C3 (Avestin)

• Preparative centrifuge Hettich RotoSilenta 630 RS - 6 x 1L

 

Protein purification:

• Cold room laboratory for biochemical investigations 

• Anaerobic glove-box for protein purification

• Liquid chromatograph FPLC AKTApurifier UPC 10 with UPC-900 UV detector and fraction collector (in anaerobic glove-box)

• Liquid preparative chromatograph FPLC NGC Quest 10 Plus system (Biorad)

• Liquid chromatograph GE AKTA Start

• Ultracentrifuges with a set of rotors (Beckman)

• High-speed centrifuge Hermle Z36HK

• Vacuum sample concentrator (Labconco-USA)

• Freeze dryer (Labconco-USA)

• Deep freezers of IPP PAS (-70°C)

• Ultra-low Freezer PLATINUM Next V -86°C

 

Analytical:

• HPLC with detection:

  • UV-Vis (200-800 nm),

  • fluorescence (200-800 nm)

  • electrochemical (COULOCHEM II).

• Agilent 1100 VL LC-MS with:

  • DAD detector,

  • Electrospray Ionization,

  • Atmospheric Pressure Chemical Ionization.

• Chromatograph with UV-VIS detector (Shimadzu) for GPC

• Agilent 1290 Infinity UHPLC with 6460 Triple Quad MS/MS detector​ (QQQ)

  • DAD detector,

  • Electrospray Ionization,

  • Atmospheric Pressure Chemical Ionization.

• Capillary electrophoresis apparatus with DAD detection (Hewlett Packard)

• Gas chromatograph Varian CP-3800 with TCD and FID 

• Bio-Rad gel electrophoresis system for proteins and DNA

• SUBMINI for fast DNA electrophoresis

• Baker Solid Phase Extraction chamber

• CFX Connect Real-Time PCR Detection System

• Microscope Olympus SZX10

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Biophysical equipment

• UV/Vis spectrophotometers with thermostatic measuring chambers:

  • Spectrophotometer UV/vis Shimadzu UV-1280 with Peltier thermostatic in the anaerobic chamber

  • Spectrophotometer UV/vis Shimadzu UV-2700 with Peltier thermostatic and RX 2000 stopped-flow module (Photophysics)

  • Epoch Microplate Spectrophotometer (BioTek Instruments)

• CFX Connect Real-Time PCR Detection System​

• Isothermal Titration Calorimeter - Thermal Activity Monitor (TAM III, Thermometric) with:

  • 4 ml Ampule Micro Calorimetric Unit,

  • 4 ml Combined Twin Ampule/Flow/Mix Micro Calorimetric Unit,

  • 4 ml Perfusion/Titration Ampule,

 

Computer modeling facilities

• Access to CYFRONET supercomputer center - PL-GRID supercomputers - calculation in Gaussian 2016, Turbomole, Amber, etc. 

• Two computer clusters consisting of servers with multiple-core AMD Opteron processors (+ NVIDIA CUDA GPU)

  • ​Gaussian 09,

  • Jaguar,

  • Molcas Programs for classical simulations: Amber, NAMD2, AutoDock, Modeller

• Dynochem software package for bioprocess modeling and scaling (Scale-Up Systems)​