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Research Topics:

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  • Oxidoreductases involved in cholesterol catabolism

  • Enzymatic substrates spectrum and reaction products

  • Steroid synthesis and biotransformation

  • Steady-state and pre-steady state kinetics

  • Enzyme-substrate complexes modelling

Methods:

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  • Aerobic and anaerobic bacteria cultivation

  • Enzyme purification with FPLC systems

  • Spectroscopic and HPLC-based enzyme activity assays

  • Stopped-flow kinetic of half-cycle redox reactions

  • Kinetic isotope effect 

  • Molecular dynamic (MD) simulations of enzyme-substrate complexes

Agnieszka Wojtkiewicz, PhD

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Member of the ICSC since: July, 2011
Phone: +48 12 6395 155,
Room: 237
E-mail: agnieszka.wojtkiewicz@ikifp.edu.pl

Agnieszka_Wojtkiewicz.jpg

ORCID: 0000-0001-7521-2233

Selected papers:

  • P. Wójcik, M. Glanowski, A. M. Wojtkiewicz, A. Rohman, M. Szaleniec, "Universal capability of 3‑ketosteroid Δ1‑dehydrogenases to catalyze Δ1‑dehydrogenation of C17‑substituted steroids", Microb. Cell Fact., 20 (2021) 119

  • A. M. Wojtkiewicz, P. Wójcik, M. Procner, M. Flejszar, M. Oszajca, M. HochoÅ‚owski, M. Tataruch, B. MrugaÅ‚a, T. Janeczko, M. Szaleniec, "The efficient Δ1-dehydrogenation of a wide spectrum of 3-ketosteroids in a broad pH range by 3-ketosteroid dehydrogenase from Sterolibacterium denitrificans", J. Steroid Biochem. Mol. Biol., 202 (2020) 105731

  • M. Tataruch, P. Wójcik, A. M. Wojtkiewicz, K. Zaczyk, K. SzymaÅ„ska, M. Szaleniec, "Application of Immobilized Cholest-4-En-3-One Δ1-Dehydrogenase from Sterolibacterium Denitrificans for Dehydrogenation of Steroids", Catalyst, 10(12) (2020) 1460

  • P. Wójcik, A. M. Wojtkiewicz, M. Tataruch, J. Morzycki, M. Szaleniec, "Sposób wytwarzania (25R)-spirosta-1,4-dien-3-onu z diosgenonu", Polish Patent Application P.433249 (13.03.2020)

  • A. Rugor, M. Szaleniec, J. StaroÅ„, "Sposób otrzymywania 25-hydroksylowanej witaminy D3 (kalcyfediolu)", Polish Patent PL 235932

  • K. SofiÅ„ska, A. M. Wojtkiewicz, P. Wójcik, O. Zastawny, M. Guzik, A. Winiarska, P. Waligórski, M. CieÅ›la, J. Barbasz, M. Szaleniec, "Investigation of quaternary structure of aggregating 3-ketosteroid dehydrogenase from Sterolibacterium denitrificans: In the pursuit of consensus of various biophysical techniques", Biochim. Biophys. Acta-Gen. Subj., 1863 (2019) 1027-1039

  • M. Malinowska, B. Miroslaw, E. Sikora, J. Ogonowski, A. M. Wojtkiewicz, M. Szaleniec, M. Pasikowska-Piwko, I. Eris, "New lupeol esters as active substances in the treatment of skin damage", PLoS ONE, 14 (2019) e0214216

  • P. Kalimuthu, A. M. Wojtkiewicz, M. Szaleniec, P. V. Bernhardt, "Electrocatalytic Hydroxylation of Sterols by Steroid C25 Dehydrogenase from Sterolibacterium denitrificans", Chem. Eur. J., 24 (30) (2018) 7710-7717

  • M. Szaleniec, A. M. Wojtkiewicz, R. Bernhardt, T. Borowski, M. Donova, "Bacterial steroid hydroxylases: enzyme classes, their functions and comparison of their catalytic mechanisms", Appl. Microbiol. Biotechnol., 102(19) (2018) 8153-8171

  • A. Rugor, M. Tataruch, J. StaroÅ„, A. Dudzik, E. Niedzialkowska, P. Nowak, A. Hogendorf, A. Michalik-Zym, D. B. Napruszewska, A. JarzÄ™bski, K. SzymaÅ„ska, W. BiaÅ‚as, M. Szaleniec, "Regioselective hydroxylation of cholecalciferol, cholesterol and other sterol derivatives by steroid C25 dehydrogenase", Appl. Microbiol. Biotechnol., 101 (2017) 1163-1174

  • E. Niedzialkowska, B. Mrugala, A. Rugor, P.C. M, A. Skotnicka, J.J. Cotelesage, G.N. George, M. Szaleniec, W. Minor, K. Lewinski,, "Optimization of overexpression of a chaperone protein of steroid C25 dehydrogenase for biochemical and biophysical characterization", Protein Expr. Purif., 134 (2017) 47-62

  • A. Rugor, A. Wójcik-Augustyn, E. Niedzialkowska, S. Mordalski, J. StaroÅ„ A. Bojarski, M. Szaleniec, "Reaction mechanism of sterol hydroxylation by steroid C25 dehydrogenase – homology model, reactivity and isoenzymatic diversity", J. Inorg. Biochem., 173 (2017) 28–43

  • M. Szaleniec, A. Rugor, A. Dudzik, M. Tataruch, K. SzymaÅ„ska, A. JarzÄ™bski, "Sposób otrzymywania 25-hydroksylowanych pochodnych sterolowych, w tym 25-hydroksy-7-dehydrocholesterolu", Polish Patent, P.411750-A1

  • A. Rugor, M. Szaleniec, T. Janeczko, M. Dymarska, E. Kostrzewa-SusÅ‚ow, "Sposób wytwarzania propionianu androst-1,4-dien-3-on-17-olu", Polish Patent P.413209

  • A. Rugor, M. Szaleniec, T. Janeczko, M. Dymarska, E. Kostrzewa-SusÅ‚ow, "Sposób wytwarzania octanu androst-1,4,6-trien-3-on-17-olu", Polish Patent P.413207

  • A. Rugor, M. Szaleniec, T. Janeczko, M. Dymarska, E. Kostrzewa-SusÅ‚ow, "Sposób wytwarzania 17a-metyloandrost-1,4-dien-3-on-17-olu", Polish Patent P.413208

  • C. Löffler, K. Kuntze, J. R. Vazquez, A. Rugor, J.W. Kung, A. Böttcher, M. Boll, "Occurrence, genes and expression of the W/Se-containing class II benzoyl-coenzyme A reductases in anaerobic bacteria", Environ. Microbiol., 13(3) (2011) 696-709

What do we do

Our aim is to combine the research potentials of Institute of Catalysis and Surface Chemistry and Institute of Plant Physiology in the field of biochemistry and biotechnology

Our Projects

Laboratory members aplly for new domestic and international projects in order to carry out increasingly advanced research

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Join our team

We are open to scientists willing to cooperate on ongoing projects as well as those coming with their own scientific ideas

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Contact us

If you are interested in research conducted in our laboratory or have any questions, please do not hesitate and feel free to contact us

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ACCESSIBILITY STATEMENT

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